Human Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) ELISA Kit | SPTLC3 elisa kit

Human Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) ELISA Kit

Cat. Num. AAA2706321

• Gene Names: SPTLC3; LCB3; SPT3; LCB 3; LCB2B; SPT 3; hLCB2b; SPTLC2L; C20orf38; dJ718P11; dJ718P11.1
• Reactivity: Human
• Synonyms: Serine Palmitoyltransferase; Long Chain Base Subunit 3 (SPTLC3); Human Serine Palmitoyltransferase; Long Chain Base Subunit 3 (SPTLC3) ELISA Kit; SPTLC2L; SPT 3; LCB 3; C20orf38; Aminotransferase 2; Serine-palmitoyl-CoA transferase 3; Long chain base biosynthesis protein 3; SPTLC3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3).; No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) and analogues was observed.
• Sequence Length: 552

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPTLC3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 223462383
• NCBI GeneID: 55304
• Molecular Weight: 19,693 Da
• NCBI Official Full Name: Serine palmitoyltransferase, long chain base subunit 3
• NCBI Official Synonym Full Names: serine palmitoyltransferase long chain base subunit 3
• NCBI Official Symbol: SPTLC3
• NCBI Official Synonym Symbols: LCB3; SPT3; LCB 3; LCB2B; SPT 3; hLCB2b; SPTLC2L; C20orf38; dJ718P11; dJ718P11.1
• NCBI Protein Information: serine palmitoyltransferase 3
• UniProt Protein Name: Serine palmitoyltransferase 3
• Protein Family: Serine palmitoyltransferase
• UniProt Gene Name: SPTLC3
• UniProt Synonym Gene Names: C20orf38; SPTLC2L; LCB2b; LCB 3; SPT 3

NCBI Description

This gene encodes a subunit of the serine palmitoyltransferase complex which catalyzes the rate-limiting step in sphingolipid biosynthesis. This subunit metabolizes lauroyl- and myristoyl-CoA and generates C14 and C16-sphingoid bases. [provided by RefSeq, Mar 2017]

Uniprot Description

SPTLC3: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.50; Endoplasmic reticulum; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 20p12.1

Cellular Component: endoplasmic reticulum membrane; integral component of membrane

Molecular Function: pyridoxal phosphate binding; serine C-palmitoyltransferase activity

Biological Process: sphingoid biosynthetic process; sphingolipid biosynthetic process