Human Seprase/FAP ELISA Kit | FAP elisa kit

Human Seprase/FAP PicoKine ELISA Kit

Cat. Num. AAA177274

• Gene Names: FAP; FAPA; DPPIV
• Reactivity: Human
• Synonyms: Seprase/FAP; Human Seprase/FAP PicoKine ELISA Kit; Seprase; fibroblast activation protein; alpha; 170 kDa melanoma membrane bound gelatinase; 170 kDa melanoma membrane-bound gelatinase; DPPIV; FAP; FAPA; Fibroblast activation protein alpha; Integral membrane serine protease; SEPR_HUMAN; FAP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Seprase
• Sequence Length: 760

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Seprase/FAP PicoKine ELISA Kit standard curve )

Related Product Information for FAP elisa kit

Principle of the assay: human Seprase ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Seprase has been precoated onto 96-well plates. Standards(NSO, L26-D760) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Seprase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Seprase amount of sample captured in plate.
Background: FAP (Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

NCBI and Uniprot Product Information

• NCBI GI #: 292495099
• NCBI GeneID: 2191
• UniProt Accession #: Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
• Molecular Weight: 26,954 Da
• NCBI Official Full Name: Seprase
• NCBI Official Synonym Full Names: fibroblast activation protein, alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; DPPIV
• NCBI Protein Information: seprase; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase
• UniProt Protein Name: Prolyl endopeptidase FAP
• Protein Family: Prolyl endopeptidase
• UniProt Gene Name: FAP
• UniProt Entry Name: SEPR_HUMAN

NCBI Description

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.26; EC 3.4.14.5; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; cell surface; focal adhesion; lamellipodium; apical part of cell; cytoplasm; plasma membrane; integral to membrane

Molecular Function: peptidase activity; integrin binding; protein dimerization activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; proteolysis; cell adhesion; regulation of fibrinolysis