Human Semaphorin 7A (SEMA7A) ELISA Kit | SEMA7A elisa kit

Human Semaphorin 7A (SEMA7A) ELISA Kit

Cat. Num. AAA2023304

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Human
• Synonyms: Semaphorin 7A (SEMA7A); Human Semaphorin 7A (SEMA7A) ELISA Kit; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL; Semaphorin 7A; GPI Membrane Anchor; John Milton Hagen Blood Group; Semaphorin-K1; Semaphorin-L; SEMA7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 7A (SEMA7A).; No significant cross-reactivity or interference between Semaphorin 7A (SEMA7A) and analogues was observed.
• Sequence Length: 652

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA7A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A (SEMA7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 7A (SEMA7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 7A (SEMA7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 7A (SEMA7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SEMA7A elisa kit

Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 225637520
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139501.1
• NCBI GenBank Nucleotide #: NM_001146029.1
• UniProt Accession #: O75326; Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5; B4DDP7; F5H1S0
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: semaphorin-7A isoform 2 preproprotein
• NCBI Official Synonym Full Names: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A
• UniProt Protein Name: Semaphorin-7A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L
• UniProt Entry Name: SEM7A_HUMAN

NCBI Description

The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q22.3-q23

Cellular Component: membrane; plasma membrane; external side of plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; osteoblast differentiation; axon guidance; axon extension; positive regulation of axon extension; regulation of inflammatory response; immune response; olfactory lobe development; positive regulation of protein amino acid phosphorylation; inflammatory response

Disease: Blood Group, John Milton Hagen System