Human Interleukin 1 Receptor Type II (IL1R2) ELISA Kit | IL1R2 elisa kit

Human Interleukin 1 Receptor Type II (IL1R2) ELISA Kit

Cat. Num. AAA2020059

• Gene Names: IL1R2; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
• Reactivity: Human
• Synonyms: Interleukin 1 Receptor Type II (IL1R2); Human Interleukin 1 Receptor Type II (IL1R2) ELISA Kit; CD121b; CD121-B; IL1-R2; IL1RB; Interleukin-1 Receptor Beta; CD121 Antigen-like Family Member B; IL1R2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Type II (IL1R2).; No significant cross-reactivity or interference between Interleukin 1 Receptor Type II (IL1R2) and analogues was observed.
• Sequence Length: 398

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Type II (IL1R2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Type II (IL1R2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1R2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type II (IL1R2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type II (IL1R2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type II (IL1R2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Type II (IL1R2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL1R2 elisa kit

Signal transduction; CD & Adhesion molecule; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4758598
• NCBI GeneID: 7850
• NCBI Accession #: NP_004624.1
• NCBI GenBank Nucleotide #: NM_004633.3
• UniProt Accession #: P27930; Q6LCE6; Q9UE68; D3DVJ5
• Molecular Weight: 33,622 Da
• NCBI Official Full Name: interleukin-1 receptor type 2 isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor, type II
• NCBI Official Symbol: IL1R2
• NCBI Official Synonym Symbols: IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
• NCBI Protein Information: interleukin-1 receptor type 2
• UniProt Protein Name: Interleukin-1 receptor type 2
• Protein Family: Interleukin-1 receptor
• UniProt Gene Name: IL1R2
• UniProt Synonym Gene Names: IL1RB; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII
• UniProt Entry Name: IL1R2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]

Uniprot Description

IL-1RB: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; interleukin-1, Type II, blocking receptor activity; interleukin-1 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; immune response