Typical Testing Data/Standard Curve (for reference only)
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit | ATP2A1 elisa kit
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ATP1A3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATP1A3 antibody and an ATP1A3-HRP conjugate. The assay sample and buffer are incubated together with ATP1A3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1A3 concentration since ATP1A3 from samples and ATP1A3-HRP conjugate compete for the anti-ATP1A3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1A3 from the sample, fewer sites are left to bind ATP1A3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1A3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
Uniprot Description
ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 3.6.3.8; Transporter; Hydrolase; Membrane protein, multi-pass; Endoplasmic reticulum; Transporter, ion channel
Chromosomal Location of Human Ortholog: 16p12.1
Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding
Biological Process: apoptotic mitochondrial changes; maintenance of mitochondrion localization; positive regulation of fast-twitch skeletal muscle contraction; metabolic process; calcium ion transport; reduction of endoplasmic reticulum calcium ion concentration; elevation of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction; blood coagulation; transmembrane transport; elevation of mitochondrial calcium ion concentration; negative regulation of striated muscle contraction
Disease: Brody Myopathy
Research Articles on ATP2A1
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Product Notes
The Human ATP2A1 atp2a1 (Catalog #AAA7230941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230941 ELISA Kit recognizes Human ATP2A1. It is sometimes possible for the material contained within the vial of "Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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