Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ELISA Kit | Atp2a1 elisa kit

Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ELISA Kit

Cat. Num. AAA763166

• Gene Names: ATP2A1; ATP2A; SERCA1
• Reactivity: Rat
• Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ELISA Kit; Atp2a1/SERCA1/SR Ca (2+)-ATPase 1/Sarcoplasmic/endoplasmic reticulum calcium ATPase 1/Endoplasmic reticulum class 1/2 Ca (2+) ATPase/Calcium pump 1/Calcium-transporting ATPase sarcoplasmic reticulum type; fast twitch skeletal muscle isoform; Atp2a1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Atp2a1. No significant cross-reactivity or interference between Atp2a1 and analogues was observed.
• Sequence Length: 994

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.312-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Atp2a1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Atp2a1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Atp2a1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Atp2a1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Atp2a1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Atp2a1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Atp2a1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10835220
• NCBI GeneID: 487
• NCBI Accession #: NP_004311.1
• NCBI GenBank Nucleotide #: NM_004320.4
• Molecular Weight: 95,199 Da
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
• NCBI Official Symbol: ATP2A1
• NCBI Official Synonym Symbols: ATP2A; SERCA1
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• Protein Family: Sarcoplasmic/endoplasmic reticulum calcium ATPase
• UniProt Gene Name: ATP2A1
• UniProt Synonym Gene Names: SERCA1; SR Ca(2+)-ATPase 1
• UniProt Entry Name: AT2A1_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]

Uniprot Description

ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral; Hydrolase; EC 3.6.3.8; Transporter; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment; I band; integral to membrane; integral to plasma membrane; membrane; mitochondrion; perinuclear region of cytoplasm; sarcoplasmic reticulum; sarcoplasmic reticulum membrane

Molecular Function: ATP binding; calcium ion binding; calcium-transporting ATPase activity; protein binding; protein homodimerization activity

Biological Process: apoptotic mitochondrial changes; blood coagulation; calcium ion transport; elevation of endoplasmic reticulum calcium ion concentration; elevation of mitochondrial calcium ion concentration; maintenance of mitochondrion localization; metabolic process; negative regulation of striated muscle contraction; positive regulation of fast-twitch skeletal muscle contraction; reduction of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction; transmembrane transport

Disease: Brody Myopathy