ELISA (EIA)
(ELISA of anti-IL-10 antibody on human rhIL-10. Binding curves of the human IgG1 chimeric version of the anti-IL-10 antibody 9D7 (MBS488592-10.0; blue line) and isotype control (MBS488034, anti-fluorescein; orange line) to an ELISA plate coated with human rhIL-10 at a concentration of 2.5ug/ml. A 3-fold serial dilution from 3000 ng/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an anti-human IgG/HRP antibody was used.)
Rat anti-Human IL-10 Monoclonal Antibody | anti-IL-10 antibody
Anti-IL-10 [9D7]
ELISA (EIA)
(ELISA of anti-IL-10 antibody on human rhIL-10. Binding curves of the human IgG1 chimeric version of the anti-IL-10 antibody 9D7 (MBS488592-10.0; blue line) and isotype control (MBS488034, anti-fluorescein; orange line) to an ELISA plate coated with human rhIL-10 at a concentration of 2.5ug/ml. A 3-fold serial dilution from 3000 ng/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an anti-human IgG/HRP antibody was used.)
ELISA (EIA)
(ELISA of anti-IL-10 antibody on human rhIL-10. Binding curves of the human IgG1 chimeric version of the anti-IL-10 antibody 9D7 (MBS488592-10.0; blue line) and isotype control (MBS488034, anti-fluorescein; orange line) to an ELISA plate coated with human rhIL-10 at a concentration of 2.5ug/ml. A 3-fold serial dilution from 3000 ng/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an anti-human IgG/HRP antibody was used.)
Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.
Note on publication: Describes the generation of antibodies aginst cytokines.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Uniprot Description
IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.
Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q31-q32
Cellular Component: extracellular space
Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding
Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; B cell proliferation; regulation of isotype switching; leukocyte chemotaxis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; negative regulation of interferon-gamma production; response to molecule of bacterial origin; positive regulation of MHC class II biosynthetic process; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; hemopoiesis; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; receptor biosynthetic process; response to activity; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion
Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on IL-10
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Product Notes
The IL-10 il10 (Catalog #AAA488593) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-10 [9D7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10 can be used in a range of immunoassay formats including, but not limited to, Neutralisation, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Crystallization. 9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002). Researchers should empirically determine the suitability of the IL-10 il10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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