Human S100 Calcium Binding Protein P (S100P) ELISA Kit | S100P elisa kit

Human S100 Calcium Binding Protein P (S100P) ELISA Kit

Cat. Num. AAA453782

• Gene Names: S100P; MIG9
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein P (S100P); Human S100 Calcium Binding Protein P (S100P) ELISA Kit; S100-P; MIG9; S100-E; Migration-inducing gene 9 protein; S100P elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100P. No significant cross-reactivity or interference between S100P and analogues was observed.
• Sequence Length: 95

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 28pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100P were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100P were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100P elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100P in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100P. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100P in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5174663
• NCBI GeneID: 6286
• NCBI Accession #: NP_005971.1
• NCBI GenBank Nucleotide #: NM_005980.2
• UniProt Accession #: P25815; Q5J7W2
• Molecular Weight: 10,400 Da
• NCBI Official Full Name: protein S100-P
• NCBI Official Synonym Full Names: S100 calcium binding protein P
• NCBI Official Symbol: S100P
• NCBI Official Synonym Symbols: MIG9
• NCBI Protein Information: protein S100-P
• UniProt Protein Name: Protein S100-P
• Protein Family: Protein
• UniProt Gene Name: S100P
• UniProt Synonym Gene Names: S100E; MIG9

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).