Human RPS19 (Ribosomal protein S19) ELISA Kit | RPS19 elisa kit

Human RPS19 (Ribosomal protein S19) ELISA Kit

Cat. Num. AAA7607191

• Gene Names: RPS19; DBA; S19; DBA1; eS19
• Reactivity: Human
• Synonyms: RPS19 (Ribosomal protein S19); Human RPS19 (Ribosomal protein S19) ELISA Kit; RPS19 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ROBO4. No significant cross-reactivity or interference between ROBO4 and analogues was observed.
• Sequence Length: 145

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ROBO4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ROBO4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPS19 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ROBO4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ROBO4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ROBO4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ROBO4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4210954
• NCBI GeneID: 6223
• Molecular Weight: 16,060 Da
• NCBI Official Full Name: ribosomal protein S19
• NCBI Official Synonym Full Names: ribosomal protein S19
• NCBI Official Symbol: RPS19
• NCBI Official Synonym Symbols: DBA; S19; DBA1; eS19
• NCBI Protein Information: 40S ribosomal protein S19
• UniProt Protein Name: 40S ribosomal protein S19
• Protein Family: Ribosomal protein
• UniProt Gene Name: RPS19

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for pre-rRNA processing and maturation of 40S ribosomal subunits.