Human Ribosomal Protein L13A (RPL13A) ELISA Kit | RPL13A elisa kit

Human Ribosomal Protein L13A (RPL13A) ELISA Kit

Cat. Num. AAA2021054

• Gene Names: RPL13A; L13A; TSTA1
• Reactivity: Human
• Synonyms: Ribosomal Protein L13A (RPL13A); Human Ribosomal Protein L13A (RPL13A) ELISA Kit; TSTA1; Tissue Specific Transplantation Antigen 1; 60S ribosomal protein L13a; 23 kDa highly basic protein; RPL13A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein L13A (RPL13A).; No significant cross-reactivity or interference between Ribosomal Protein L13A (RPL13A) and analogues was observed.
• Sequence Length: 203

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein L13A (RPL13A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPL13A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein L13A (RPL13A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein L13A (RPL13A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein L13A (RPL13A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein L13A (RPL13A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RPL13A elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6552365
• NCBI GeneID: 23521
• UniProt Accession #: P40429; A8K505
• Molecular Weight: 23,577 Da
• NCBI Official Full Name: ribosomal protein L13a
• NCBI Official Synonym Full Names: ribosomal protein L13a
• NCBI Official Symbol: RPL13A
• NCBI Official Synonym Symbols: L13A; TSTA1
• NCBI Protein Information: 60S ribosomal protein L13a
• UniProt Protein Name: 60S ribosomal protein L13a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL13A
• UniProt Entry Name: RL13A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

RPL13A: Belongs to the ribosomal protein L13P family.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol; nucleus; ribonucleoprotein complex; large ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; negative regulation of translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination