Human Ribosomal Protein L13A (RPL13A) RTU ELISA Kit | RPL13A rtu elisa kit

Human Ribosomal Protein L13A (RPL13A) ELISA Kit

Cat. Num. AAA4502282

• Gene Names: RPL13A; L13A; TSTA1
• Reactivity: Human
• Synonyms: Ribosomal Protein L13A (RPL13A); Human Ribosomal Protein L13A (RPL13A) ELISA Kit; TSTA1; Tissue Specific Transplantation Antigen 1; 60S ribosomal protein L13a; 23 kDa highly basic protein; RPL13A rtu elisa kit

• Reactivity: Human
• Sequence Length: 142

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPL13A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPL13A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RPL13A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RPL13A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RPL13A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 395132450
• NCBI GeneID: 23521
• NCBI Accession #: NP_001257420.1
• NCBI GenBank Nucleotide #: NM_001270491.1
• UniProt Accession #: P40429; A8K505
• Molecular Weight: 23,577 Da
• NCBI Official Full Name: 60S ribosomal protein L13a isoform 2
• NCBI Official Synonym Full Names: ribosomal protein L13a
• NCBI Official Symbol: RPL13A
• NCBI Official Synonym Symbols: L13A; TSTA1
• NCBI Protein Information: 60S ribosomal protein L13a
• UniProt Protein Name: 60S ribosomal protein L13a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL13A

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.