Human Renal Tumor Antigen (RAGE) ELISA Kit | RAGE elisa kit

Human Renal Tumor Antigen (RAGE) ELISA Kit

Cat. Num. AAA454839

• Gene Names: MOK; RAGE; RAGE1; STK30; RAGE-1
• Reactivity: Human
• Synonyms: Renal Tumor Antigen (RAGE); Human Renal Tumor Antigen (RAGE) ELISA Kit; MOK RAGE1 MAPK/MAK/MRK Overlapping Kinase; RAGE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAGE. No significant cross-reactivity or interference between RAGE and analogues was observed.
• Sequence Length: 389

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.066ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAGE were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAGE were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAGE elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RAGE in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAGE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 436408722
• NCBI GeneID: 5891
• NCBI Accession #: NP_001258940.1
• NCBI GenBank Nucleotide #: NM_001272011.1
• UniProt Accession #: Q9UQ07; Q92790; Q93067; B2R6Z4; B7Z7P6; E7ER76; E7ERR8
• Molecular Weight: 47,886 Da
• NCBI Official Full Name: MAPK/MAK/MRK overlapping kinase isoform 2
• NCBI Official Synonym Full Names: MOK protein kinase
• NCBI Official Symbol: MOK
• NCBI Official Synonym Symbols: RAGE; RAGE1; STK30; RAGE-1
• NCBI Protein Information: MAPK/MAK/MRK overlapping kinase
• UniProt Protein Name: MAPK/MAK/MRK overlapping kinase
• UniProt Gene Name: MOK
• UniProt Synonym Gene Names: RAGE; RAGE1; RAGE-1

NCBI Description

This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.