Western Blot (WB)
(Western blot analysis of PGP9.5 expression in SH-SY5Y cell lysate.)
Rabbit PGP9.5 Polyclonal Antibody | anti-PGP9.5 antibody
Anti-PGP9.5 Rabbit pAb
Gene Names
UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
PGP9.5; Polyclonal Antibody; Anti-PGP9.5 Rabbit pAb; anti-PGP9.5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PGP9.5 Antibody detects endogenous levels of total PGP9.5
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
223
Applicable Applications for anti-PGP9.5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
Immunogen
A synthesized peptide derived from human PGP9.5
Subcellular Location
Endoplasmic reticulum membrane; Lipid-anchor
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of PGP9.5 expression in SH-SY5Y cell lysate.)
Western Blot (WB)
(Western blot analysis of PGP9.5 expression in SH-SY5Y cell lysate.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain, using PGP9.5 Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain, using PGP9.5 Antibody.)
Related Product Information for anti-PGP9.5 antibody
PGP9.5 is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). Hydrolyze isopeptide bonds between the carboxy-terminal glycine of Ub and the epsilon-amino group of lysine on target proteins and involved in the cotranslational processing of pro-ubiquitin and ribosomal proteins translated as ubiquitin fusions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
NCBI Description
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Uniprot Description
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Research Articles on PGP9.5
Similar Products
Product Notes
The PGP9.5 uchl1 (Catalog #AAA4750678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGP9.5 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGP9.5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50. Researchers should empirically determine the suitability of the PGP9.5 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP9.5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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