Typical Testing Data/Standard Curve (for reference only)
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Human Ras Homolog Gene Family, Member A (RHOA) ELISA Kit | RHOA elisa kit
Human Ras Homolog Gene Family, Member A (RHOA) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to RHOA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RHOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RHOA, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RHOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Uniprot Description
RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
Protein type: Oncoprotein; G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: apical junction complex; cytoskeleton; focal adhesion; lamellipodium; plasma membrane; midbody; cell cortex; cell junction; cytosol; cleavage furrow
Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding
Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; phosphoinositide-mediated signaling; positive regulation of I-kappaB kinase/NF-kappaB cascade; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of neuron differentiation
Research Articles on RHOA
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Product Notes
The Human RHOA rhoa (Catalog #AAA2023112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023112 ELISA Kit recognizes Human RHOA. It is sometimes possible for the material contained within the vial of "Ras Homolog Gene Family, Member A (RHOA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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