CYB5R1 recombinant protein

CYB5R1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121341

• Gene Names: CYB5R1; B5R1; B5R2; B5R.1; NQO3A2; humb5R2
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CYB5R1; CYB5R1 Protein; Human; Recombinant (His Tag); Human CYB5R1 Protein (His Tag); B5R.1 Protein; B5R1 Protein; B5R2 Protein; humb5R2 Protein; NQO3A2 Protein; PRO9865 Protein; UNQ3049 Protein; cytochrome b5 reductase 1; CYB5R1 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu29-Tyr305

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CYB5R1 (Q9UHQ9)(Leu 29-Tyr 305) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

Troncoso C, et al. (2008) Influence of electron transport proteins on the reactions catalyzed by Fusarium fujikuroi gibberellin monooxygenases. Phytochemistry. 69(3): 672-83.Qian W, et al. (1998) The influence of mutation at Glu44 and Glu56 of cytochrome b5 on the protein's stabilization and interaction between cytochrome c and cytochrome b5. Biochemistry. 37 (40): 14137-50.Zhu H, et al. (2000) Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells. Proc Natl Acad Sci. 96 (26): 14742-7.

NCBI and Uniprot Product Information

• NCBI GI #: 49574502
• NCBI GeneID: 51706
• NCBI Accession #: NP_057327.2
• NCBI GenBank Nucleotide #: NM_016243.2
• UniProt Accession #: Q9UHQ9; O95329; Q53F73; Q8NCL5; Q9UHJ1; A0PK21; B2R8E0
• Molecular Weight: 34,095 Da
• NCBI Official Full Name: NADH-cytochrome b5 reductase 1
• NCBI Official Synonym Full Names: cytochrome b5 reductase 1
• NCBI Official Symbol: CYB5R1
• NCBI Official Synonym Symbols: B5R1; B5R2; B5R.1; NQO3A2; humb5R2
• NCBI Protein Information: NADH-cytochrome b5 reductase 1; NAD(P)H:quinone oxidoreductase type 3, polypeptide A2
• UniProt Protein Name: NADH-cytochrome b5 reductase 1
• Protein Family: NADH-cytochrome b5 reductase
• UniProt Gene Name: CYB5R1
• UniProt Synonym Gene Names: NQO3A2; b5R.1
• UniProt Entry Name: NB5R1_HUMAN

Uniprot Description

CYB5R1: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Protein type: Oxidoreductase; EC 1.6.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: membrane; mitochondrion; integral to membrane

Molecular Function: cytochrome-b5 reductase activity

Biological Process: sterol biosynthetic process