Human Raft Linking Protein (Raftlin) ELISA Kit | RFTN1 elisa kit

Human Raft Linking Protein (Raftlin) ELISA Kit

Cat. Num. AAA2122103

• Reactivity: Human
• Synonyms: Raft Linking Protein (Raftlin); Human Raft Linking Protein (Raftlin) ELISA Kit; RFTN1; MIG2; Raftlin; Lipid Raft Linker 1; Cell migration-inducing gene 2 protein; RFTN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Raft Linking Protein (Raftlin).; No significant cross-reactivity or interference between Raft Linking Protein (Raftlin) and analogues was observed.
• Sequence Length: 578

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.113ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Raft Linking Protein (Raftlin) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Raft Linking Protein (Raftlin) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RFTN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Raft Linking Protein (Raftlin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Raft Linking Protein (Raftlin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Raft Linking Protein (Raftlin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Raft Linking Protein (Raftlin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RFTN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 41872577
• NCBI Accession #: NP_055965.1
• NCBI GenBank Nucleotide #: NP_055965.1
• UniProt Accession #: Q14699
• NCBI Official Full Name: raftlin
• UniProt Protein Name: Raftlin
• Protein Family: Raftlin
• UniProt Gene Name: RFTN1
• UniProt Synonym Gene Names: KIAA0084
• UniProt Entry Name: RFTN1_HUMAN

Uniprot Description

RFTN1: May play a pivotal role in the formation and/or maintenance of lipid rafts. May regulate B-cell antigen receptor- mediated signaling. Belongs to the raftlin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: protein complex; cytoplasm; plasma membrane; endosome; lipid raft

Molecular Function: double-stranded RNA binding

Biological Process: protein transport into lipid raft; B cell receptor signaling pathway; response to exogenous dsRNA; T cell antigen processing and presentation; interleukin-17 production; dsRNA transport; toll-like receptor 3 signaling pathway; lipid raft formation; T cell receptor signaling pathway; growth