Human Puromycin Sensitive Aminopeptidase (PSA) ELISA Kit | PSA elisa kit

Human Puromycin Sensitive Aminopeptidase (PSA) ELISA Kit

Cat. Num. AAA454594

• Gene Names: NPEPPS; PSA; AAP-S; MP100
• Reactivity: Human
• Synonyms: Puromycin Sensitive Aminopeptidase (PSA); Human Puromycin Sensitive Aminopeptidase (PSA) ELISA Kit; NPEPPS; AAP-S; Metalloproteinase MP100; Cytosol alanyl aminopeptidase; PSA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSA. No significant cross-reactivity or interference between PSA and analogues was observed.
• Sequence Length: 915

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PSA in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1057503108
• NCBI GeneID: 9520
• NCBI Accession #: NP_001317186.1
• NCBI GenBank Nucleotide #: NM_001330257.1
• UniProt Accession #: P55786; Q6P145; Q9NP16; Q9UEM2; B7Z463
• Molecular Weight: 94,516 Da
• NCBI Official Full Name: puromycin-sensitive aminopeptidase isoform 2
• NCBI Official Synonym Full Names: aminopeptidase puromycin sensitive
• NCBI Official Symbol: NPEPPS
• NCBI Official Synonym Symbols: PSA; AAP-S; MP100
• NCBI Protein Information: puromycin-sensitive aminopeptidase
• UniProt Protein Name: Puromycin-sensitive aminopeptidase
• UniProt Gene Name: NPEPPS
• UniProt Synonym Gene Names: PSA; PSA; AAP-S

NCBI Description

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain.