Human PTPRS (Receptor-type tyrosine-protein phosphatase S) ELISA Kit | PTPRS elisa kit

Human PTPRS (Receptor-type tyrosine-protein phosphatase S) ELISA Kit

Cat. Num. AAA7608127

• Gene Names: PTPRS; R-PTP-S; PTPSIGMA; R-PTP-sigma
• Reactivity: Human
• Synonyms: PTPRS (Receptor-type tyrosine-protein phosphatase S); Human PTPRS (Receptor-type tyrosine-protein phosphatase S) ELISA Kit; Receptor-type tyrosine-protein phosphatase S; R-PTP-S; Receptor-type tyrosine-protein phosphatase sigma; R-PTP-sigma; PTPRS; PTPRS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PTPRS. No significant cross-reactivity or interference between PTPRS and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTPRS elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20809883
• NCBI GeneID: 5802
• UniProt Accession #: Q13332; O75255; O75870; Q15718; Q16341; Q2M3R7
• Molecular Weight: 215,980 Da
• NCBI Official Full Name: PTPRS protein
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type S
• NCBI Official Symbol: PTPRS
• NCBI Official Synonym Symbols: R-PTP-S; PTPSIGMA; R-PTP-sigma
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase S
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase S
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRS
• UniProt Synonym Gene Names: R-PTP-S; R-PTP-sigma

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycan (PubMed:21454754). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth. Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection. Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as tyrosine phosphatase (PubMed:8524829). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120).