Rabbit anti-Mouse TFIIIC90 Polyclonal Antibody | anti-TFIIIC90 antibody

TFIIIC90, NT (GTF3C4, General transcription factor 3C polypeptide 4, TF3C-delta, Transcription factor IIIC 90kD subunit, Transcription factor IIIC subunit delta) (Azide free) (HRP)

Cat. Num. AAA6355111

• Gene Names: GTF3C4; KAT12; TFIII90; TFIIIC90; TFIIIC290; TFIIICDELTA
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TFIIIC90; Polyclonal Antibody; NT (GTF3C4; General transcription factor 3C polypeptide 4; TF3C-delta; Transcription factor IIIC 90kD subunit; Transcription factor IIIC subunit delta) (Azide free) (HRP); GTF3C4; Transcription factor IIIC subunit delta; anti-TFIIIC90 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TFIIIC90 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-252 amino acids from the N-terminal region of human TFIIIC90.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TFIIIC90 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TFIIIC90 antibody detected the TFIIIC90 protein (arrow).)

Flow Cytometry (FC/FACS)

(TFIIIC90 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TFIIIC90 antibody

Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.

Product Categories/Family for anti-TFIIIC90 antibody

Antibodies; Transcription Factors

References

Joubert, B.R., et al. Genome Med 2 (3), 17 (2010): Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 156119605
• NCBI GeneID: 9329
• NCBI Accession #: NP_036336
• NCBI GenBank Nucleotide #: NM_012204
• UniProt Accession #: Q9UKN8; Q5VZJ7
• Molecular Weight: 91,982 Da
• NCBI Official Full Name: general transcription factor 3C polypeptide 4
• NCBI Official Synonym Full Names: general transcription factor IIIC, polypeptide 4, 90kDa
• NCBI Official Symbol: GTF3C4
• NCBI Official Synonym Symbols: KAT12; TFIII90; TFIIIC90; TFIIIC290; TFIIICDELTA
• NCBI Protein Information: general transcription factor 3C polypeptide 4; TF3C-delta; TFIIIC 90 kDa subunit; general transcription factor 3C 4; transcription factor IIIC 90 kDa subunit; transcription factor IIIC subunit delta
• UniProt Protein Name: General transcription factor 3C polypeptide 4
• UniProt Gene Name: GTF3C4
• UniProt Synonym Gene Names: TFIIIC 90 kDa subunit; TFIIIC90
• UniProt Entry Name: TF3C4_HUMAN

Uniprot Description

GTF3C4: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. Belongs to the TFIIIC subunit 4 family.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: nucleoplasm; transcription factor TFIIIC complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; enzyme activator activity

Biological Process: transcription from RNA polymerase III promoter; positive regulation of catalytic activity; transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; 5S class rRNA transcription; gene expression; histone acetylation; tRNA transcription from RNA polymerase III promoter