Typical Testing Data/Standard Curve (for reference only)
Human PTGS1 ELISA Kit | PTGS1 elisa kit
Human PTGS1 (Prostaglandin Endoperoxide Synthase 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PTGS1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PTGS1. You can calculate the concentration of Human PTGS1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
COX-1: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.14.99.1; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 9q32-q33.3
Cellular Component: endoplasmic reticulum membrane; photoreceptor outer segment; intracellular membrane-bound organelle; cytoplasm; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; dioxygenase activity; metal ion binding; heme binding
Biological Process: regulation of blood pressure; xenobiotic metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; lipid metabolic process; response to oxidative stress; inflammatory response; prostaglandin biosynthetic process; regulation of cell proliferation
Research Articles on PTGS1
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Product Notes
The Human PTGS1 ptgs1 (Catalog #AAA2504341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504341 ELISA Kit recognizes Human PTGS1. It is sometimes possible for the material contained within the vial of "PTGS1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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