Human protein tyrosine kinase (PTK) ELISA Kit | PTK elisa kit

Human protein tyrosine kinase (PTK) ELISA Kit

Cat. Num. AAA269536

• Gene Names: BLK; MODY11
• Reactivity: Human
• Synonyms: protein tyrosine kinase (PTK); Human protein tyrosine kinase (PTK) ELISA Kit; PTK elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 505

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTK elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PTK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PTK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS.Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 914204
• NCBI GeneID: 640
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: protein tyrosine kinase
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK
• UniProt Entry Name: BLK_HUMAN

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; innate immune response; positive regulation of insulin secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11