Canine Protein tyrosine kinase ELISA Kit | PTK elisa kit

Canine Protein tyrosine kinase ELISA Kit

Cat. Num. AAA755607

• Gene Names: BLK; MODY11
• Reactivity: Canine
• Synonyms: Protein tyrosine kinase; Canine Protein tyrosine kinase ELISA Kit; PTK elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTK elisa kit

Principle of the assay: PTK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTK antibody and an PTK-HRP conjugate. The assay sample and buffer are incubated together with PTK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTK concentration since PTK from samples and PTK-HRP conjugate compete for the anti-PTK antibody binding site. Since the number of sites is limited, as more sites are occupied by PTK from the sample, fewer sites are left to bind PTK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 914204
• NCBI GeneID: 640
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: protein tyrosine kinase
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK
• UniProt Entry Name: BLK_HUMAN

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; positive regulation of insulin secretion; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of cell proliferation

Disease: Maturity-onset Diabetes Of The Young, Type 11