Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein SSX2 (SSX2) Standard Curve.)
Human Protein SSX2 (SSX2) ELISA Kit | SSX2 elisa kit
Human Protein SSX2 (SSX2) ELISA Kit
Reactivity
Human
Synonyms
Protein SSX2 (SSX2); Human Protein SSX2 (SSX2) ELISA Kit; SSX2; RP11-552J9.2; CT5.2a; HD21; HOM-MEL-40; MGC119055; MGC15364; MGC3884; SSX; cancer/testis antigen family 5; member 2a; sarcoma; synovial; X-chromosome-related 2; synovial sarcoma; X breakpoint 2; isofo; SSX2 elisa kit
Reactivity
Human
Specificity
Human Protein SSX2 (SSX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSX2. No significant cross-reactivity or interference between Human SSX2 and analogues was observed.
Sequence Length
188
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein SSX2 (SSX2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein SSX2 (SSX2) Standard Curve.)
Related Product Information for SSX2 elisa kit
Description: This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2 in samples. An antibody specific for SSX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SSX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SSX2 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
Background: SSX2 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
NCBI and Uniprot Product Information
Uniprot Description
SSX2B: Could act as a modulator of transcription. A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency). Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: nucleus
Molecular Function: protein binding; nucleic acid binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Similar Products
Product Notes
The Human SSX2 ssx2 (Catalog #AAA9714038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714038 ELISA Kit recognizes Human SSX2. It is sometimes possible for the material contained within the vial of "Protein SSX2 (SSX2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
