Human Protein C (PC) ELISA Kit | PC elisa kit

Human Protein C (PC) ELISA Kit

Cat. Num. AAA287502

• Gene Names: PROC; PC; APC; PROC1; THPH3; THPH4
• Reactivity: Human
• Synonyms: Protein C (PC); Human Protein C (PC) ELISA Kit; APC; PC; PROC; anticoagulant protein C; autoprothrombin IIA; blood coagulation factor XIV; protein C; vitamin K-dependent protein C; PC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PROC. No significant cross-reactivity or interference between Human PROC and analogues was observed.
• Sequence Length: 461

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PC elisa kit

This assay employs a two-site sandwich ELISA to quantitate PROC in samples. An antibody specific for PROC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4506115
• NCBI GeneID: 5624
• NCBI Accession #: NP_000303.1
• NCBI GenBank Nucleotide #: NM_000312.3
• UniProt Accession #: P04070; Q15189; Q15190; Q16001; Q53S74; Q9UC55; B4DPQ7
• Molecular Weight: 57,556 Da
• NCBI Official Full Name: vitamin K-dependent protein C preproprotein
• NCBI Official Synonym Full Names: protein C, inactivator of coagulation factors Va and VIIIa
• NCBI Official Symbol: PROC
• NCBI Official Synonym Symbols: PC; APC; PROC1; THPH3; THPH4
• NCBI Protein Information: vitamin K-dependent protein C
• UniProt Protein Name: Vitamin K-dependent protein C
• UniProt Gene Name: PROC

NCBI Description

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Uniprot Description

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).