Human Protamine 2 (PRM2) ELISA Kit | PRM2 elisa kit

Human Protamine 2 (PRM2) ELISA Kit

Cat. Num. AAA456137

• Gene Names: PRM2; CT94.2
• Reactivity: Human
• Synonyms: Protamine 2 (PRM2); Human Protamine 2 (PRM2) ELISA Kit; PRM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRM2. No significant cross-reactivity or interference between PRM2 and analogues was observed.
• Sequence Length: 140

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRM2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRM2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PRM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PRM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PRM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 556503330
• NCBI GeneID: 5620
• NCBI Accession #: NP_001273285.1
• NCBI GenBank Nucleotide #: NM_001286356.1
• UniProt Accession #: P04554; Q6ZMM0
• Molecular Weight: 17,106 Da
• NCBI Official Full Name: protamine-2 isoform 2
• NCBI Official Synonym Full Names: protamine 2
• NCBI Official Symbol: PRM2
• NCBI Official Synonym Symbols: CT94.2
• NCBI Protein Information: protamine-2
• UniProt Protein Name: Protamine-2
• UniProt Gene Name: PRM2
• UniProt Synonym Gene Names: P2'

NCBI Description

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis, and are the major DNA-binding proteins in the nucleus of sperm in many vertebrates. They package the sperm DNA into a highly condensed complex in a volume less than 5% of a somatic cell nucleus. Many mammalian species have only one protamine (protamine 1); however, a few species, including human and mouse, have two. This gene encodes protamine 2, which is cleaved to give rise to a family of protamine 2 peptides. Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.