Human Prostatic Acid Phosphatase (ACPP) ELISA Kit | ACPP elisa kit

Human Prostatic Acid Phosphatase (ACPP) ELISA Kit

Cat. Num. AAA9714548

• Reactivity: Human
• Synonyms: Prostatic Acid Phosphatase (ACPP); Human Prostatic Acid Phosphatase (ACPP) ELISA Kit; ACPP; ACP-3; ACP3; PAP; prostatic acid phosphotase; ACPP elisa kit

• Reactivity: Human
• Specificity: Human Prostatic acid phosphatase (ACPP) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACPP. No significant cross-reactivity or interference between Human ACPP and analogues was observed.
• Sequence Length: 387

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Prostatic acid phosphatase (ACPP) Standard Curve.)

Related Product Information for ACPP elisa kit

Description: This Human Prostatic acid phosphatase (ACPP) ELISA Kit employs a two-site sandwich ELISA to quantitate ACPP in samples. An antibody specific for ACPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Prostatic acid phosphatase (PAP) is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases.The highest levels of acid phosphatase are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels.Certain medications can cause temporary increases or decreases in acid phosphatase levels. Manipulation of the prostate gland through massage, biopsy or rectal exam before a test may increase the level.Its physiological function may be associated with the liquefaction process of semen.

NCBI and Uniprot Product Information

• NCBI GI #: 149643069
• NCBI GeneID: 504700
• NCBI Accession #: NP_001092336.1
• NCBI GenBank Nucleotide #: NP_001092336.1
• UniProt Accession #: A6H730
• NCBI Official Full Name: prostatic acid phosphatase
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Protein Information: prostatic acid phosphatase
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Prostatic acid phosphatase
• UniProt Gene Name: ACPP
• UniProt Entry Name: PPAP_BOVIN

Uniprot Description

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma ().