Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acid phosphatase-like protein 2 (ACPL2) Standard Curve.)
Rat Acid Phosphatase-Like Protein 2 (ACPL2) ELISA Kit | ACPL2 elisa kit
Rat Acid Phosphatase-Like Protein 2 (ACPL2) ELISA Kit
Gene Names
Pxylp1; Acpl2
Reactivity
Rat
Synonyms
Acid Phosphatase-Like Protein 2 (ACPL2); Rat Acid Phosphatase-Like Protein 2 (ACPL2) ELISA Kit; ACPL2; FLJ23751; ACPL2 elisa kit
Reactivity
Rat
Specificity
Rat Acid phosphatase-like protein 2 (ACPL2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ACPL2. No significant cross-reactivity or interference between Rat ACPL2 and analogues was observed.
Sequence Length
480
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acid phosphatase-like protein 2 (ACPL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Acid phosphatase-like protein 2 (ACPL2) Standard Curve.)
Related Product Information for ACPL2 elisa kit
Description: This Rat Acid phosphatase-like protein 2 (ACPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACPL2 in samples. An antibody specific for ACPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ACPL2 belongs to the histidine acid phosphatase family. Catalyses the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum. Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function; therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer.
Background: ACPL2 belongs to the histidine acid phosphatase family. Catalyses the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum. Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function; therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
2-phosphoxylose phosphatase 1
NCBI Official Synonym Full Names
2-phosphoxylose phosphatase 1
NCBI Official Symbol
Pxylp1
NCBI Official Synonym Symbols
Acpl2
NCBI Protein Information
2-phosphoxylose phosphatase 1
UniProt Protein Name
2-phosphoxylose phosphatase 1
UniProt Gene Name
Pxylp1
Uniprot Description
Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.
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Product Notes
The Rat ACPL2 pxylp1 (Catalog #AAA9711954) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711954 ELISA Kit recognizes Rat ACPL2. It is sometimes possible for the material contained within the vial of "Acid Phosphatase-Like Protein 2 (ACPL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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