Human Prostaglandin D2 Synthase, Brain (PGD2S) ELISA Kit | PGD2S elisa kit

Human Prostaglandin D2 Synthase, Brain (PGD2S) ELISA Kit

Cat. Num. AAA455255

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Reactivity: Human
• Synonyms: Prostaglandin D2 Synthase; Brain (PGD2S); Human Prostaglandin D2 Synthase; Brain (PGD2S) ELISA Kit; REG1-A; REG; PSP; ICRF; PSPS; PSPS1; PTP; Lithostathine 1 Alpha; Pancreatic Stone Protein;; PGD2S elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGD2S. No significant cross-reactivity or interference between PGD2S and analogues was observed.
• Sequence Length: 190

• Samples: Serum, plasma, cerebrospinal fluid and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 26.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGD2S were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGD2S were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for PGD2S elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PGD2S in human serum, plasma, cerebrospinal fluid and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGD2S. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PGD2S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PGD2S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PGD2S in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32171249
• NCBI GeneID: 5730
• NCBI Accession #: NP_000945.3
• NCBI GenBank Nucleotide #: NM_000954.5
• UniProt Accession #: A0A024R8G3; P41222
• Molecular Weight: 21,029 Da
• NCBI Official Full Name: prostaglandin-H2 D-isomerase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase
• UniProt Protein Name: Prostaglandin D2 synthase 21kDa (Brain), isoform CRA_a
• UniProt Gene Name: PTGDS

NCBI Description

The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]