Rabbit anti-Human PRLHR Polyclonal Antibody | anti-PRLHR antibody

PRLHR, CT (PRLHR, GPR10, GR3, Prolactin-releasing peptide receptor, G-protein coupled receptor 10, hGR3) (Azide free) (HRP)

Cat. Num. AAA6337483

• Gene Names: PRLHR; GR3; GPR10; PrRPR
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRLHR; Polyclonal Antibody; CT (PRLHR; GPR10; GR3; Prolactin-releasing peptide receptor; G-protein coupled receptor 10; hGR3) (Azide free) (HRP); hGR3; anti-PRLHR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRLHR antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRLHR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-312 amino acids from the C-terminal region of human PRLHR.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PRLHR Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PRLHR antibody detected the PRLHR protein (arrow).)

Related Product Information for anti-PRLHR antibody

PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).

Product Categories/Family for anti-PRLHR antibody

Antibodies; G Protein Receptors

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Lagerstrom, M.C., et al. Genomics 85(6):688-703(2005). Ellacott, K.L., et al. Peptides 26(4):675-681(2005). Deloukas, P., et al. Nature 429(6990):375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4758474
• NCBI GeneID: 2834
• NCBI Accession #: NP_004239.1
• NCBI GenBank Nucleotide #: NM_004248.2
• UniProt Accession #: P49683; O75194; Q502U8; Q5VXR9
• Molecular Weight: 41,121 Da
• NCBI Official Full Name: prolactin-releasing peptide receptor
• NCBI Official Synonym Full Names: prolactin releasing hormone receptor
• NCBI Official Symbol: PRLHR
• NCBI Official Synonym Symbols: GR3; GPR10; PrRPR
• NCBI Protein Information: prolactin-releasing peptide receptor; hGR3; prRP receptor; G protein-coupled receptor 10; G-protein coupled receptor 10; prolactin releasing peptide receptor; prolactin-releasing hormone receptor
• UniProt Protein Name: Prolactin-releasing peptide receptor
• Protein Family: Prolactin-releasing peptide receptor
• UniProt Gene Name: PRLHR
• UniProt Synonym Gene Names: GPR10; GR3; PrRP receptor; PrRPR
• UniProt Entry Name: PRLHR_HUMAN

NCBI Description

PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).[supplied by OMIM, Mar 2008]

Uniprot Description

GPR10: Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; neuropeptide receptor activity; neuropeptide Y receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; hormone metabolic process; feeding behavior; female pregnancy