Human Probable ATP-dependent RNA helicase DHX40 ELISA Kit | DHX40 elisa kit

Human Probable ATP-dependent RNA helicase DHX40 (DHX40) ELISA Kit

Cat. Num. AAA282148

• Gene Names: DHX40; PAD; DDX40; ARG147
• Reactivity: Human
• Synonyms: Probable ATP-dependent RNA helicase DHX40; Human Probable ATP-dependent RNA helicase DHX40 (DHX40) ELISA Kit; DHX40 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DHX40. No significant cross-reactivity or interference between Human DHX40 and analogues was observed.
• Sequence Length: 779

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DHX40 elisa kit

DHX40 is a member of the DEAH box family of ATP-dependent RNA helicases. RNA helicases catalyze the unwinding of double-stranded RNA and play a role in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and organellar gene expression.Xu et al. (2002) cloned DHX40, which they called DDX40, during large-scale cDNA sequencing of clones obtained from a fetal brain cDNA library. The deduced 779-amino acid protein has a calculated molecular mass of 86 kD. DHX40 has a well-conserved DEAH box region and shares high amino acid identity with DHX8 and the yeast proteins Prp22 and Dhr1, both of which function in RNA processing. Northern blot analysis detected ubiquitous expression of a 3.6-kb DHX40 transcript.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX40 in samples. An antibody specific for DHX40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX40 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 261878481
• NCBI GeneID: 79665
• NCBI Accession #: NP_001159773.1
• NCBI GenBank Nucleotide #: NM_001166301.1
• UniProt Accession #: Q8IX18; Q5JPH4; Q8TC86; Q8WY53; Q9BXM1; Q9H6M9; B3KTJ5; C9JR60
• Molecular Weight: 80,221 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DHX40 isoform 2
• NCBI Official Synonym Full Names: DEAH-box helicase 40
• NCBI Official Symbol: DHX40
• NCBI Official Synonym Symbols: PAD; DDX40; ARG147
• NCBI Protein Information: probable ATP-dependent RNA helicase DHX40
• UniProt Protein Name: Probable ATP-dependent RNA helicase DHX40
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DHX40
• UniProt Synonym Gene Names: DDX40

NCBI Description

This gene encodes a member of the DExH/D box family of ATP-dependent RNA helicases that have an essential role in RNA metabolism. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 17.[provided by RefSeq, Oct 2009]

Uniprot Description

DHX40: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: cytoplasm; spliceosomal complex

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding

Biological Process: mRNA splicing, via spliceosome