Typical Testing Data/Standard Curve (for reference only)
Human Probable ATP dependent RNA helicase DDX46 (DDX46) ELISA Kit | DDX46 elisa kit
Human Probable ATP dependent RNA helicase DDX46 (DDX46) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DDX46 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DDX46 antibody and an DDX46-HRP conjugate. The assay sample and buffer are incubated together with DDX46-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX46 concentration since DDX46 from samples and DDX46-HRP conjugate compete for the anti-DDX46 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX46 from the sample, fewer sites are left to bind DDX46-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX46 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
Uniprot Description
DDX46: Plays an essential role in splicing, either prior to, or during splicing A complex formation. Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.
Protein type: EC 3.6.4.13; Spliceosome; Helicase
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: Cajal body; nuclear speck; nucleus
Molecular Function: helicase activity; ATP binding
Biological Process: RNA splicing; mRNA processing
Research Articles on DDX46
Similar Products
Product Notes
The Human DDX46 ddx46 (Catalog #AAA7241461) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7241461 ELISA Kit recognizes Human DDX46. It is sometimes possible for the material contained within the vial of "Probable ATP dependent RNA helicase DDX46 (DDX46), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
