Typical Testing Data/Standard Curve (for reference only)
Human ATP dependent RNA helicase DDX3Y (DDX3Y) ELISA Kit | DDX3Y elisa kit
Human ATP dependent RNA helicase DDX3Y (DDX3Y) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DDX3Y ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DDX3Y antibody and an DDX3Y-HRP conjugate. The assay sample and buffer are incubated together with DDX3Y-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX3Y concentration since DDX3Y from samples and DDX3Y-HRP conjugate compete for the anti-DDX3Y antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX3Y from the sample, fewer sites are left to bind DDX3Y-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX3Y concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
DDX3Y: a Y-linked DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DBY mutations causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Two alternatively spliced transcripts differ only in the length of the 3' UTR.
Protein type: RNA-binding; EC 3.6.4.13; Helicase
Chromosomal Location of Human Ortholog: Yq11
Cellular Component: membrane; cytoplasm; nucleus
Molecular Function: DNA binding; RNA binding; helicase activity; ATP binding
Biological Process: metabolic process
Disease: Spermatogenic Failure, Y-linked, 2
Research Articles on DDX3Y
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Product Notes
The Human DDX3Y ddx3y (Catalog #AAA7232428) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232428 ELISA Kit recognizes Human DDX3Y. It is sometimes possible for the material contained within the vial of "ATP dependent RNA helicase DDX3Y (DDX3Y), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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