Human Probable ATP-dependent RNA helicase DDX4 ELISA Kit | DDX4 elisa kit

Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit

Cat. Num. AAA280921

• Gene Names: DDX4; VASA
• Reactivity: Human
• Synonyms: Probable ATP-dependent RNA helicase DDX4; Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit; DDX4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DDX4. No significant cross-reactivity or interference between Human DDX4 and analogues was observed.
• Sequence Length: 704

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DDX4 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX4 in samples. An antibody specific for DDX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 216548263
• NCBI GeneID: 54514
• NCBI Accession #: NP_001136021.1
• NCBI GenBank Nucleotide #: NM_001142549.1
• UniProt Accession #: Q9NQI0; Q5M7Z3; Q86VX0; Q9NT92; Q9NYB1; A8K8Q2; B3KSF4; D6RDK4; E9PCD8
• Molecular Weight: 77,056 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX4 isoform 2
• NCBI Official Synonym Full Names: DEAD-box helicase 4
• NCBI Official Symbol: DDX4
• NCBI Official Synonym Symbols: VASA
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX4
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX4
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX4
• UniProt Synonym Gene Names: VASA

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

DDX4: May play a role in germ cell development. May play a role in sperm motility. Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Belongs to the DEAD box helicase family. DDX4/VASA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; nucleolus; perinuclear region of cytoplasm

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; ATPase activity; nucleic acid binding

Biological Process: cell differentiation; DNA methylation during gametogenesis; male meiosis I; male meiotic nuclear division; multicellular organism development; RNA secondary structure unwinding; RNA-mediated gene silencing; sperm motility; spermatogenesis