Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit | PTBP1 elisa kit

Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit

Cat. Num. AAA744316

• Gene Names: PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• Reactivity: Human
• Synonyms: Polypyrimidine Tract Binding Protein 1 (PTBP1); Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit; PTBP1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This PLD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLD. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTBP1 elisa kit

Principle of the Assay: PLD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLD antibody and an PLD-HRP conjugate. The assay sample and buffer are incubated together with PLD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLD concentration since PLD from samples and PLD-HRP conjugate compete for the anti-PLD antibody binding site. Since the number of sites is limited, as more sites are occupied by PLD from the sample, fewer sites are left to bind PLD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLD concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14165466
• NCBI GeneID: 5725
• NCBI Accession #: NP_114368.1
• NCBI GenBank Nucleotide #: NM_031991.3
• UniProt Accession #: P26599; Q9BUQ0
• Molecular Weight: 57,221 Da
• NCBI Official Full Name: polypyrimidine tract-binding protein 1 isoform c
• NCBI Official Synonym Full Names: polypyrimidine tract binding protein 1
• NCBI Official Symbol: PTBP1
• NCBI Official Synonym Symbols: PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• NCBI Protein Information: polypyrimidine tract-binding protein 1; hnRNP I; RNA-binding protein; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I)
• UniProt Protein Name: Polypyrimidine tract-binding protein 1
• Protein Family: Polypyrimidine tract-binding protein
• UniProt Gene Name: PTBP1
• UniProt Synonym Gene Names: PTB; PTB; hnRNP I
• UniProt Entry Name: PTBP1_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; membrane; nucleolus

Molecular Function: protein binding; poly-pyrimidine tract binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of muscle cell differentiation; RNA splicing; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome