Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arachidonate-5-Lipoxygenase (ALOX5) Standard Curve.)
Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit | ALOX5 elisa kit
Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arachidonate-5-Lipoxygenase (ALOX5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Arachidonate-5-Lipoxygenase (ALOX5) Standard Curve.)
Background: Arachidonate 5-lipoxygenase is a member of the lipoxygenase family. It transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.5-ALOX catalyzes oxidation of AA at the 5-position to yield 5-HPETE. 5-LO then converts 5-HPETE to leukotriene A4.5-ALOX is a target for pharmaceutical intervention in CAD.Some people with variant alleles for 5-LO are at elevated risk for CAD. 5-ALOX is expressed in brain cells and may participate in neuropathologic processes.Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
NCBI and Uniprot Product Information
NCBI Description
catalyzes the conversion of arachidonate to leukotriene A4 in leukotriene metabolism [RGD, Feb 2006]
Uniprot Description
5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.
Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Nuclear envelope; Lipid Metabolism - linoleic acid; Oxidoreductase
Cellular Component: cytoplasm; cytosol; dendrite; extracellular space; nuclear envelope; nuclear envelope lumen; nuclear matrix; nuclear membrane; nucleus; sarcolemma
Molecular Function: arachidonate 5-lipoxygenase activity; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding
Biological Process: acute inflammatory response; arachidonic acid metabolic process; inflammatory response; leukotriene biosynthetic process; leukotriene metabolic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; positive regulation of vasoconstriction; response to hyperoxia; response to nutrient; sensory perception of pain
Research Articles on ALOX5
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Product Notes
The Human ALOX5 alox5 (Catalog #AAA9716364) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716364 ELISA Kit recognizes Human ALOX5. It is sometimes possible for the material contained within the vial of "Arachidonate-5-Lipoxygenase (ALOX5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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