Human Plasminogen Activator, Tissue (tPA) ELISA Kit | tPA elisa kit

Human Plasminogen Activator, Tissue (tPA) ELISA Kit

Cat. Num. AAA454524

• Gene Names: PLAT; TPA; T-PA
• Reactivity: Human
• Synonyms: Plasminogen Activator; Tissue (tPA); Human Plasminogen Activator; Tissue (tPA) ELISA Kit; PLAT T-PA t-plasminogen activator Alteplase Reteplase; tPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TPA. No significant cross-reactivity or interference between TPA and analogues was observed.
• Sequence Length: 562

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 6.7pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for tPA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TPA in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505861
• NCBI GeneID: 5327
• NCBI Accession #: NP_000921.1
• NCBI GenBank Nucleotide #: NM_000930.4
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• Molecular Weight: 44,373 Da
• NCBI Official Full Name: tissue-type plasminogen activator isoform 1 preproprotein
• NCBI Official Synonym Full Names: plasminogen activator, tissue type
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Taurine--pyruvate aminotransferase
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.