Human Placenta Specific Protein 9 (PLAC9) ELISA Kit | PLAC9 elisa kit

Human Placenta Specific Protein 9 (PLAC9) ELISA Kit

Cat. Num. AAA2707271

• Reactivity: Human
• Synonyms: Placenta Specific Protein 9 (PLAC9); Human Placenta Specific Protein 9 (PLAC9) ELISA Kit; PLAC9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Placenta Specific Protein 9 (PLAC9).; No significant cross-reactivity or interference between Placenta Specific Protein 9 (PLAC9) and analogues was observed.
• Sequence Length: 97

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.28ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Specific Protein 9 (PLAC9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Specific Protein 9 (PLAC9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLAC9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Specific Protein 9 (PLAC9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Specific Protein 9 (PLAC9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Specific Protein 9 (PLAC9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Specific Protein 9 (PLAC9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 61175222
• NCBI GeneID: 219348
• NCBI Accession #: NP_001012991.1
• NCBI GenBank Nucleotide #: NM_001012973.1
• UniProt Accession #: Q5JTB6
• Molecular Weight: 10,309 Da
• NCBI Official Full Name: placenta-specific protein 9 isoform 1
• NCBI Official Synonym Full Names: placenta specific 9
• NCBI Official Symbol: PLAC9
• NCBI Protein Information: placenta-specific protein 9
• UniProt Protein Name: Placenta-specific protein 9
• Protein Family: Placenta-specific protein
• UniProt Gene Name: PLAC9