Mouse Opioid Receptor Kappa 1 (OPRk1) ELISA Kit | OPRk1 elisa kit

Mouse Opioid Receptor Kappa 1 (OPRk1) ELISA Kit

Cat. Num. AAA2704885

• Gene Names: OPRK1; KOR; OPRK; KOR-1; K-OR-1
• Reactivity: Mouse
• Synonyms: Opioid Receptor Kappa 1 (OPRk1); Mouse Opioid Receptor Kappa 1 (OPRk1) ELISA Kit; OPR-K; KOR; OPRk1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Opioid Receptor Kappa 1 (OPRk1).; No significant cross-reactivity or interference between Opioid Receptor Kappa 1 (OPRk1) and analogues was observed.
• Sequence Length: 380

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.28ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Opioid Receptor Kappa 1 (OPRk1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Opioid Receptor Kappa 1 (OPRk1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPRk1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Opioid Receptor Kappa 1 (OPRk1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Opioid Receptor Kappa 1 (OPRk1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Opioid Receptor Kappa 1 (OPRk1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Opioid Receptor Kappa 1 (OPRk1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119607129
• NCBI GeneID: 4986
• Molecular Weight: 33,319 Da
• NCBI Official Full Name: opioid receptor, kappa 1
• NCBI Official Synonym Full Names: opioid receptor kappa 1
• NCBI Official Symbol: OPRK1
• NCBI Official Synonym Symbols: KOR; OPRK; KOR-1; K-OR-1
• NCBI Protein Information: kappa-type opioid receptor
• UniProt Protein Name: Kappa-type opioid receptor
• Protein Family: Kappa-type opioid receptor
• UniProt Gene Name: OPRK1
• UniProt Synonym Gene Names: OPRK; K-OR-1; KOR-1

NCBI Description

This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]

Uniprot Description

KOR-1: kappa-type opioid receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8q11.23

Cellular Component: axon terminus; dendrite; integral component of membrane; integral component of plasma membrane; neuron projection; perikaryon; plasma membrane

Molecular Function: dynorphin receptor activity; neuropeptide binding; opioid receptor activity; protein binding

Biological Process: adenylate cyclase-inhibiting opioid receptor signaling pathway; behavior; behavioral response to cocaine; conditioned place preference; defense response to virus; eating behavior; G-protein coupled receptor signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; immune response; locomotory behavior; maternal behavior; negative regulation of luteinizing hormone secretion; neuropeptide signaling pathway; opioid receptor signaling pathway; phospholipase C-activating G-protein coupled receptor signaling pathway; positive regulation of dopamine secretion; positive regulation of locomotion; positive regulation of p38MAPK cascade; positive regulation of potassium ion transmembrane transport; regulation of energy homeostasis; regulation of saliva secretion; regulation of sensory perception of pain; response to acrylamide; response to estrogen; response to ethanol; response to insulin stimulus; response to morphine; response to radiation; sensory perception; sensory perception of pain; sensory perception of temperature stimulus; synaptic transmission