Human pituitary adenylate cyclase activating polypeptide, PACAP ELISA Kit | PACAP elisa kit

Human pituitary adenylate cyclase activating polypeptide, PACAP ELISA Kit

Cat. Num. AAA160511

• Gene Names: ADCYAP1; PACAP
• Reactivity: Human
• Synonyms: pituitary adenylate cyclase activating polypeptide; PACAP; Human pituitary adenylate cyclase activating polypeptide; PACAP ELISA Kit; PACAP elisa kit

• Reactivity: Human
• Sequence Length: 176

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.3-90ng/ml
• Sensitivity: 0.14ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PACAP elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human pituitary adenylate cyclase activating polypeptide (also known as PACAP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PACAP antibody. PACAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PACAP Antibody is added and binds to PACAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PACAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PACAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

References

Pituitary adenylate cyclase–activating polypeptide is reduced in Alzheimer disease. Pengcheng Han, MD, PhD, Winnie Liang, PhD, Leslie C. Baxter, PhD, Junxiang Yin, MD, PhD, Zhiwei Tang, MD, PhD, Thomas G. Beach, MD, Richard J. Caselli, MD, Eric M. Reiman, MD and Jiong Shi, MD, PhD (2014)
Pituitary adenylate cyclase-activating polypeptide protects against ?-amyloid toxicity. Pengcheng Han, Zhiwei Tang, Junxiang Yin, Marwan Maalouf, Thomas G. Beach, Eric M. Reiman, Jiong Shi (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 245806
• NCBI GeneID: 116
• UniProt Accession #: P18509; Q52LQ0; B2R7N4
• Molecular Weight: 18,835 Da
• NCBI Official Full Name: pituitary adenylate cyclase activating polypeptide
• NCBI Official Synonym Full Names: adenylate cyclase activating polypeptide 1
• NCBI Official Symbol: ADCYAP1
• NCBI Official Synonym Symbols: PACAP
• NCBI Protein Information: pituitary adenylate cyclase-activating polypeptide
• UniProt Protein Name: Pituitary adenylate cyclase-activating polypeptide
• UniProt Gene Name: ADCYAP1
• UniProt Synonym Gene Names: PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

NCBI Description

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

Uniprot Description

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells ().