Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-3 (M6PRBP1) Standard Curve.)
Human Perilipin-3 (M6PRBP1) ELISA Kit | PLIN3 elisa kit
Human Perilipin-3 (M6PRBP1) ELISA Kit
Gene Names
PLIN3; PP17; TIP47; M6PRBP1
Reactivity
Human
Synonyms
Perilipin-3 (M6PRBP1); Human Perilipin-3 (M6PRBP1) ELISA Kit; M6PRBP1; MGC11117; MGC2012; PP17; TIP47; MPR-binding protein 47 kDa; cargo selection protein (mannose 6 phosphate receptor binding protein); cargo selection protein TIP47; mannose 6 phosphate receptor; PLIN3 elisa kit
Reactivity
Human
Specificity
Human Perilipin-3 (M6PRBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human M6PRBP1. No significant cross-reactivity or interference between Human M6PRBP1 and analogues was observed.
Sequence Length
433
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-3 (M6PRBP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-3 (M6PRBP1) Standard Curve.)
Related Product Information for PLIN3 elisa kit
Description: This Human Perilipin-3 (M6PRBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate M6PRBP1 in samples. An antibody specific for M6PRBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M6PRBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M6PRBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M6PRBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by PLIN3 interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for PLIN3.
Background: Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by PLIN3 interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for PLIN3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
perilipin-3 isoform 2
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
NCBI Protein Information
perilipin-3
UniProt Protein Name
Perilipin-3
Protein Family
UniProt Gene Name
PLIN3
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17
UniProt Entry Name
PLIN3_HUMAN
NCBI Description
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
Research Articles on PLIN3
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Product Notes
The Human PLIN3 plin3 (Catalog #AAA9716033) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716033 ELISA Kit recognizes Human PLIN3. It is sometimes possible for the material contained within the vial of "Perilipin-3 (M6PRBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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