Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) ELISA Kit | PNPLA2 elisa kit

Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) ELISA Kit

Cat. Num. AAA2706529

• Gene Names: PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• Reactivity: Human
• Synonyms: Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2); Human Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) ELISA Kit; ATGL; PEDF-R; TTS2; Desnutrin; Pigment epithelium-derived factor; Transport-secretion protein 2; Adipose Triglyceride Lipase; Calcium-independent phospholipase A2; PNPLA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2).; No significant cross-reactivity or interference between Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) and analogues was observed.
• Sequence Length: 180

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.128ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PNPLA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Patatin Like Phospholipase Domain Containing Protein 2 (PNPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32698724
• NCBI GeneID: 57104
• NCBI Accession #: NP_065109.1
• NCBI GenBank Nucleotide #: NM_020376.3
• UniProt Accession #: Q96AD5; O60643; Q5EFF5; Q6XYE5; Q96ET6; Q9NQ61; Q9NQ62
• Molecular Weight: 19,875 Da
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 2
• NCBI Official Synonym Full Names: patatin like phospholipase domain containing 2
• NCBI Official Symbol: PNPLA2
• NCBI Official Synonym Symbols: ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 2
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 2
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA2
• UniProt Synonym Gene Names: ATGL; TTS2

NCBI Description

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

Uniprot Description

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.