Human Parvovirus B19 IgG ELISA Kit
Parvovirus B19 IgG ELISA
Reactivity
Human
Synonyms
Parvovirus B19 IgG; Parvovirus B19 IgG ELISA; Parvovirus B19 IgG elisa kit
Reactivity
Human
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. It is 100%.
Samples
Human serum and plasma (edta, lithium heparin or citrate plasma)
Intra-assay Precision
The intra-assay (within-run) precision of the ELISA was determined by 20x measurements of 12 samples covering the measuring range of the ELISA.
Inter-assay Precision
The inter-assay variation of the ELISA was determined in 20 independent runs with 2 rep-licates per run.
Related Product Information for Parvovirus B19 IgG elisa kit
Intended Uses: The Parvovirus B19 IgG (Recombinant) Enzyme Immunoassay Kit provides materials for the determination of IgG-class antibodies to Parvovirus B19 in human serum and plasma (EDTA, lithium heparin or citrate plasma).
Principle of the Assay: The Parvovirus B19 IgG ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) Micro-titer wells as a solid phase are coated with recombinant Parvovirus B19 antigen (VP1-s proteins). Di-luted samples and ready-for-use controls are pipetted into these wells. During incubation Parvovirus B19-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate, the immune complexes formed (in case of posi-tive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19-specific IgG antibody in the sample. Optical density at 450 nm is read using an ELISA microtiter plate reader.
Principle of the Assay: The Parvovirus B19 IgG ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) Micro-titer wells as a solid phase are coated with recombinant Parvovirus B19 antigen (VP1-s proteins). Di-luted samples and ready-for-use controls are pipetted into these wells. During incubation Parvovirus B19-specific antibodies of positive samples and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate, the immune complexes formed (in case of posi-tive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19-specific IgG antibody in the sample. Optical density at 450 nm is read using an ELISA microtiter plate reader.
Product Categories/Family for Parvovirus B19 IgG elisa kit
Similar Products
Product Notes
The Human Parvovirus B19 IgG (Catalog #AAA494069) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494069 ELISA Kit recognizes Human Parvovirus B19 IgG. It is sometimes possible for the material contained within the vial of "Parvovirus B19 IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
