Human Pannexin 1 (PANX1) ELISA Kit | PANX1 elisa kit

Human Pannexin 1 (PANX1) ELISA Kit

Cat. Num. AAA2024821

• Gene Names: PANX1; PX1; MRS1; UNQ2529
• Reactivity: Human
• Synonyms: Pannexin 1 (PANX1); Human Pannexin 1 (PANX1) ELISA Kit; MRS1; PX1; Innexin; PANX1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pannexin 1 (PANX1).; No significant cross-reactivity or interference between Pannexin 1 (PANX1) and analogues was observed.
• Sequence Length: 422

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pannexin 1 (PANX1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pannexin 1 (PANX1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PANX1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pannexin 1 (PANX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pannexin 1 (PANX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pannexin 1 (PANX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pannexin 1 (PANX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PANX1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 15193205
• NCBI GeneID: 24145
• Molecular Weight: 47,635 Da
• NCBI Official Full Name: pannexin 1
• NCBI Official Synonym Full Names: pannexin 1
• NCBI Official Symbol: PANX1
• NCBI Official Synonym Symbols: PX1; MRS1; UNQ2529
• NCBI Protein Information: pannexin-1
• UniProt Protein Name: Pannexin-1
• Protein Family: Pannexin
• UniProt Gene Name: PANX1
• UniProt Synonym Gene Names: MRS1
• UniProt Entry Name: PANX1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. [provided by RefSeq, Jul 2008]

Uniprot Description

PANX1: Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis. Belongs to the pannexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: endoplasmic reticulum membrane; protein complex; membrane; endoplasmic reticulum; integral to membrane; gap junction; plasma membrane

Molecular Function: actin filament binding; protease binding; leak channel activity; protein heterodimerization activity; calcium channel activity; gap junction hemi-channel activity; receptor binding

Biological Process: positive regulation of interleukin-1 alpha secretion; synaptic transmission; calcium ion transport; innate immune response; positive regulation of interleukin-1 beta secretion; cation transport; response to ATP