Mouse Cardiac Troponin I  Antibody | anti-TNNI3 antibody

Anti-Cardiac Troponin I  Antibody

Cat. Num. AAA7607317

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Protein A+G Purification
• Synonyms: Cardiac Troponin I ; Anti-Cardiac Troponin I Antibody; Cardiac troponin I; cTnI; RCM1; TNNC1; TNNI3; troponin I type 3(cardiac); Troponin I; cardiac muscle; anti-TNNI3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G Purification
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 210

• Applicable Applications for anti-TNNI3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:5000-1:50000; IHC: 1:50-1:500; IF: 1:50-1:500
• Immunogen: Troponin I type 3(cardiac)
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue slide using MBS7607317 (Cardiac Troponin I Antibody) at dilution of 1:600)

Immunofluorescence (IF)

(Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MBS7607317 (TNNI3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L))

Western Blot (WB)

(Rat heart tissue were subjected to SDS PAGE followed by western blot with MBS7607317 (Cardiac Troponin Iantibody) at dilution of 1:10000)

Related Product Information for anti-TNNI3 antibody

Troponin I(TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I(cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction(AMI) for decades. Detection of cTnI in serum is widely accepted as a tool in acute coronary syndromes.

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429
• Molecular Weight: 72 kDa
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I3, cardiac type
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a