Human Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit | PLUNC elisa kit

Human Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit

Cat. Num. AAA2706386

• Gene Names: BPIFA1; LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
• Reactivity: Human
• Synonyms: Palate/Lung And Nasal Epithelium Associated Protein (PLUNC); Human Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit; BPIFA1; SPURT; NASG; LUNX; SPLUNC1; Secretory Protein In Upper Respiratory Tracts; BPI Fold Containing Family A; Member 1; Nasopharyngeal carcinoma-related; PLUNC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLUNC. No significant cross-reactivity or interference between PLUNC and analogues was observed.
• Sequence Length: 242

• Samples: Human serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: <5.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLUNC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLUNC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLUNC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLUNC in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLUNC. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLUNC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLUNC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLUNC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114319015
• NCBI GeneID: 51297
• Molecular Weight: 25,257 Da
• NCBI Official Full Name: palate lung and nasal epithelium carcinoma associated protein
• NCBI Official Synonym Full Names: BPI fold containing family A member 1
• NCBI Official Symbol: BPIFA1
• NCBI Official Synonym Symbols: LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
• NCBI Protein Information: BPI fold-containing family A member 1
• UniProt Protein Name: BPI fold-containing family A member 1
• UniProt Gene Name: BPIFA1
• UniProt Synonym Gene Names: LUNX; NASG; PLUNC; SPLUNC1; SPURT; SPLUNC1

NCBI Description

This gene is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The encoded antimicrobial protein displays antibacterial activity against Gram-negative bacteria. It is thought to be involved in inflammatory responses to irritants in the upper airways and may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only three are known. [provided by RefSeq, Aug 2014]

Uniprot Description

Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.