Human Dopamine decarboxylase ELISA Kit | DDC elisa kit

Human Dopamine decarboxylase ELISA Kit

Cat. Num. AAA726048

• Gene Names: DDC; AADC
• Reactivity: Human
• Synonyms: Dopamine decarboxylase; Human Dopamine decarboxylase ELISA Kit; DDC elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ug/mL
• Sensitivity: 1.0ug/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDC elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This DDA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DDA. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY DDA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDA antibody and an DDA-HRP conjugate. The assay sample and buffer are incubated together with DDA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDA concentration since DDA from samples and DDA-HRP conjugate compete for the anti-DDA antibody binding site. Since the number of sites is limited, as more sites are occupied by DDA from the sample, fewer sites are left to bind DDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DDC elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 48145565
• NCBI GeneID: 1644
• UniProt Accession #: Q6IBS8; P20711
• Molecular Weight: 53,880 Da
• NCBI Official Full Name: DDC
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: DDC protein
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Entry Name: Q6IBS8_HUMAN

NCBI Description

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Lyase; Amino Acid Metabolism - tyrosine; Nuclear receptor co-regulator; Amino Acid Metabolism - tryptophan; EC 4.1.1.28; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: synaptic vesicle; cell soma; axon; cytosol

Molecular Function: amino acid binding; protein domain specific binding; aromatic-L-amino-acid decarboxylase activity; protein binding; enzyme binding; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; circadian rhythm; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid

Disease: Aromatic L-amino Acid Decarboxylase Deficiency