Testing Data
(IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)
Mouse MAP1LC3A Monoclonal Antibody | anti-MAP1LC3A antibody
MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 l
WB: 1:100
ICC: 1:50-100
Applications are based on unconjugated antibody.
Testing Data
(IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)
Testing Data
(IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)
Western Blot (WB)
(Western blot analysis using antibody MBS642917 at 8ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).)
Western Blot (WB)
(Western blot analysis using antibody MBS642917 at 8ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).)
Western Blot (WB)
(Western blot analysis of rapamycin and bafilomycin- reated Hela cells. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.)
Western Blot (WB)
(Western blot analysis of rapamycin and bafilomycin- reated Hela cells. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.)
Immunohistochemistry (IHC)
(10X (lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.)
Immunohistochemistry (IHC)
(10X (lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.)
NCBI and Uniprot Product Information
NCBI Description
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
Uniprot Description
LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Microtubule-binding; Ubiquitin-like modifier; Autophagy; Vesicle
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding
Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation
Research Articles on MAP1LC3A
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Product Notes
The MAP1LC3A map1lc3a (Catalog #AAA6121375) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 l reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP1LC3A can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FLISA: 1:1,000 WB: 1:100 ICC: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAP1LC3A map1lc3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP1LC3A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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