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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Paired box protein Pax-9 (PAX9) Standard Curve.)

Human Paired Box Protein Pax-9 (PAX9) ELISA Kit | PAX9 elisa kit

Human Paired Box Protein Pax-9 (PAX9) ELISA Kit

Gene Names
PAX9; STHAG3
Reactivity
Human
Synonyms
Paired Box Protein Pax-9 (PAX9); Human Paired Box Protein Pax-9 (PAX9) ELISA Kit; PAX9; STHAG3; paired domain gene 9; PAX9 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Paired box protein Pax-9 (PAX9) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAX9. No significant cross-reactivity or interference between Human PAX9 and analogues was observed.
Sequence Length
331
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Paired box protein Pax-9 (PAX9) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Paired box protein Pax-9 (PAX9) Standard Curve.)
Related Product Information for PAX9 elisa kit
Description: This Human Paired box protein Pax-9 (PAX9) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX9 in samples. An antibody specific for PAX9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAX9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: PAX9 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 9 gene is unknown but it may involve development of stratified squamous epithelia as well as various organs and skeletal elements.Stapleton et al. (1993) isolated a cosmid for PAX9, a novel member of the paired box-containing gene family. They found it to be closely related in its paired domain to PAX1. Wallin et al. (1993) independently cloned the Pax9 gene in the mouse. PAX9 is a member of the same subgroup as Pax1/undulated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Pax9, partial
NCBI Official Synonym Full Names
paired box 9
NCBI Official Symbol
PAX9
NCBI Official Synonym Symbols
STHAG3
NCBI Protein Information
paired box protein Pax-9
UniProt Protein Name
Paired box protein Pax-9
Protein Family
UniProt Gene Name
PAX9
UniProt Entry Name
PAX9_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. Mice lacking this gene exhibit impaired development of organs, musculature and the skeleton, including absent and abnormally developed teeth, and neonatal lethality. Mutations in the human gene are associated with selective tooth agenesis-3. [provided by RefSeq, Sep 2015]

Uniprot Description

PAX9: Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs. Interacts with KDM5B.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 14q13.3

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: odontogenesis; transcription, DNA-dependent; regulation of odontogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; endoderm development

Disease: Tooth Agenesis, Selective, 3

Research Articles on PAX9

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Product Notes

The Human PAX9 pax9 (Catalog #AAA9714966) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714966 ELISA Kit recognizes Human PAX9. It is sometimes possible for the material contained within the vial of "Paired Box Protein Pax-9 (PAX9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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