Human P-Selectin Glycoprotein Ligand 1 (PSGL1) ELISA Kit | PSGL1 elisa kit

Human P-Selectin Glycoprotein Ligand 1 (PSGL1) ELISA Kit

Cat. Num. AAA2025547

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Synonyms: P-Selectin Glycoprotein Ligand 1 (PSGL1); Human P-Selectin Glycoprotein Ligand 1 (PSGL1) ELISA Kit; CD162; SELPLG; CLA; Selectin P Ligand; PSGL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of P-Selectin Glycoprotein Ligand 1 (PSGL1).; No significant cross-reactivity or interference between P-Selectin Glycoprotein Ligand 1 (PSGL1) and analogues was observed.
• Sequence Length: 412

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.254ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P-Selectin Glycoprotein Ligand 1 (PSGL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P-Selectin Glycoprotein Ligand 1 (PSGL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSGL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to P-Selectin Glycoprotein Ligand 1 (PSGL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-Selectin Glycoprotein Ligand 1 (PSGL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P-Selectin Glycoprotein Ligand 1 (PSGL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PSGL1 elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 331284236
• NCBI GeneID: 6404
• NCBI Accession #: NP_002997.2
• NCBI GenBank Nucleotide #: NM_003006.4
• Molecular Weight: 44,648 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 2
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell adhesion; blood coagulation; leukocyte tethering or rolling; leukocyte migration; leukocyte adhesive activation