Human OSBPL8 ELISA Kit | OSBPL8 elisa kit

Human OSBPL8 (Oxysterol Binding Protein Like Protein 8) ELISA Kit

Cat. Num. AAA2515657

• Gene Names: OSBPL8; ORP8; MST120; OSBP10; MSTP120
• Reactivity: Human
• Synonyms: OSBPL8; Human OSBPL8 (Oxysterol Binding Protein Like Protein 8) ELISA Kit; OSBPL8 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanOSBPL8. No significant cross-reactivity or interference between HumanOSBPL8 and analogues was observed.
• Sequence Length: 728

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSBPL8 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanOSBPL8concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OSBPL8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OSBPL8and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OSBPL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OSBPL8.You can calculate the concentration of OSBPL8in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51895890
• NCBI GeneID: 114882
• Molecular Weight: 96,956 Da
• NCBI Official Full Name: OSBPL8 protein, partial
• NCBI Official Synonym Full Names: oxysterol binding protein-like 8
• NCBI Official Symbol: OSBPL8
• NCBI Official Synonym Symbols: ORP8; MST120; OSBP10; MSTP120
• NCBI Protein Information: oxysterol-binding protein-related protein 8; ORP-8; OSBP-related protein 8
• UniProt Protein Name: Oxysterol-binding protein-related protein 8
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL8
• UniProt Synonym Gene Names: KIAA1451; ORP8; OSBP10; ORP-8; OSBP-related protein 8
• UniProt Entry Name: OSBL8_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

OSBPL8: a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two splice variant isoforms have been described.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: nuclear membrane; membrane

Molecular Function: cholesterol binding

Biological Process: fat cell differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of insulin receptor signaling pathway; lipid transport; negative regulation of cell migration